Development and Application of Quantitative Detection Method for Viral Hemorrhagic Septicemia Virus (VHSV) Genogroup IVa
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چکیده
منابع مشابه
Development and Application of Quantitative Detection Method for Viral Hemorrhagic Septicemia Virus (VHSV) Genogroup IVa
Viral hemorrhagic septicemia virus (VHSV) is a problematic pathogen in olive flounder (Paralichthys olivaceus) aquaculture farms in Korea. Thus, it is necessary to develop a rapid and accurate diagnostic method to detect this virus. We developed a quantitative RT-PCR (qRT-PCR) method based on the nucleocapsid (N) gene sequence of Korean VHSV isolate (Genogroup IVa). The slope and R² values of t...
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A recombinant viral hemorrhagic septicemia virus (rVHSV-deltaNV-EGFP) containing the enhanced green fluorescent protein (EGFP) gene instead of the NV gene was produced using the reverse-genetics method. For use as a positive control, another recombinant virus (rVHSV-wild) was also generated, which had an identical nucleotide sequence to the wild-type VHSV genome except for a few artificially re...
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Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases of farmed rainbow trout, in Turkey, wh...
متن کاملGenetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey
Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases in farmed rainbow trout, in Turkey, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trou...
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Viral hemorrhagic septicemia virus (VHSV) is an OIE-listed pathogen of fish, recently expanding in known host and geographic range in North America. Through a group process designed for subjective probability assessment, an international panel of fish health experts identified and weighted risk factors perceived important to the emergence and spread of the viral genotype, VHSV IVb, within and f...
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ژورنال
عنوان ژورنال: Viruses
سال: 2014
ISSN: 1999-4915
DOI: 10.3390/v6052204